Team 08: Diversification of muscle and heart cells in normal and pathological conditions

Web Site

There is growing evidence that the molecular mechanisms controlling early developmental events have been largely conserved between invertebrate to vertebrate species. Based on this assumption, Drosophila appears as an excellent model organism to study functions and interactions of conserved genes. Our research group is using Drosophila model to study molecular mechanisms that control diversification of muscle and cardiac cells during normal development and in pathological contexts. Our main goal is to apply the power of the Drosophila model and to develop cell-specific genomic approaches for analyzing genetic control of diversification processes.

Research

During last years using genome-wide strategies we identified the first target genes of cell identity factors Ladybird and determined the first molecular code of acting downstream realisator genes. In parallel we also defined signaling events that are required for specification Drosophila muscle stem cells and a gene cascade governing morphogenesiss of the cardiac outflow. Our recent work (in collaboration with U. Straehle, KIT Karlsruhe) has also led to the identification of developmental functions of metabolic genes and in particular of those encoding glycolytic enzymes.

Another axis of our research is dedicated to build Drosophila models for human muscular diseases. Within this frame we have generated a new Drosophila model of Myotonic Dystrophy type 1 (DM1) and performed phenotypic and transcriptomic analyses.

A part of our team works actually on technological projects funded by the Infrastructure grant TEFOR and is involved in platform activities.

Altogether research projects of the team are dedicated to:

  • Fundamental research : “Studying diversification of cardiac and muscle cells using genome wide cell specific approaches »
  • Applied research : « Generation of Drosophila models for human muscular diseases »
  • Platform activities (www.fly-facility.com): « Genome editing and invalidation of genes via Talens technologies and generation and phenotyping of collection transgenic Drosophila lines for intersectional approaches »

Research thematics

People

Last Name First Name Position Contact
Benjamin BERTIN profile picture BERTIN Benjamin Ph.D Student
Nathanaelle DANGLADES profile picture DANGLADES Nathanaelle Intern
Jean-Philippe DA PONTE profile picture DA PONTE Jean-Philippe Research Technician
Cristiana DONDI profile picture DONDI Cristiana Research Fellow
Krzysztof JAGLA profile picture JAGLA Krzysztof Principal Investigator
Teresa JAGLA profile picture JAGLA Teresa Research Engineer
Guillaume JUNION profile picture JUNION Guillaume Research Fellow
Lilia LADDADA profile picture LADDADA Lilia Ph.D Student
Quentin LAURICHESSE profile picture LAURICHESSE Quentin Ph.D Student
Guillaume LAVERGNE profile picture LAVERGNE Guillaume Ph.D Student
Thomas MOREAU profile picture MOREAU Thomas Post-doctoral Fellow
Cedric SOLER profile picture SOLER Cedric Associate Professor
Monika ZMOJDZIAN profile picture ZMOJDZIAN Monika Post-doctoral Fellow

Publications

  • 2016
    • C. Soler, L. Laddada and K. Jagla, “Coordinated Development of Muscles and Tendon-Like Structures: Early Interactions in the Drosophila Leg.”, Frontiers in physiology, vol. 7 , pp. 22, 2016.
  • 2015
  • 2014
    • R. Deplus, L. Blanchon, A. Rajavelu, A. Boukaba, M. Defrance, J. Luciani, F. Rothe, S. Dedeurwaerder, H. Denis, A. Brinkman, F. Simmer, F. Muller, B. Bertin, M. Berdasco, P. Putmans, E. Calonne, D. Litchfield, Y. de Launoit, T. Jurkowski, H. Stunnenberg, C. Bock, C. Sotiriou, M. Fraga, M. Esteller, A. Jeltsch and F. Fuks, “Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a.”, Cell Rep, vol. 8 (3) , pp. 743–53, 2014.
    • O. Taghli-Lamallem, K. Jagla, J. Chamberlain and R. Bodmer, “Mechanical and non-mechanical functions of Dystrophin can prevent cardiac abnormalities in Drosophila.”, Experimental gerontology, vol. 49 , pp. 26–34, 2014.
  • 2013
    • V. Tixier, L. Bataille, C. Etard, T. Jagla, M. Weger, J. Daponte, U. Strahle, T. Dickmeis and K. Jagla, “Glycolysis supports embryonic muscle growth by promoting myoblast fusion.”, Proc. Natl. Acad. Sci. U.S.A., vol. 110 (47) , pp. 18982–7, 2013.
    • A. Mbodj, G. Junion, C. Brun, E. Furlong and D. Thieffry, “Logical modelling of Drosophila signalling pathways.”, Mol Biosyst, vol. 9 (9) , pp. 2248–58, 2013.
    • M. Zmojdzian and K. Jagla, “Tailup plays multiple roles during cardiac outflow assembly in Drosophila.”, Cell Tissue Res., vol. 354 (2) , pp. 639–45, 2013.
    • L. Picchio, E. Plantie, Y. Renaud, P. Poovthumkadavil and K. Jagla, “Novel Drosophila model of myotonic dystrophy type 1: phenotypic characterization and genome-wide view of altered gene expression.”, Hum. Mol. Genet., vol. 22 (14) , pp. 2795–810, 2013.
  • 2012
    • G. Junion, M. Spivakov, C. Girardot, M. Braun, E. Gustafson, E. Birney and E. Furlong, “A transcription factor collective defines cardiac cell fate and reflects lineage history.”, Cell, vol. 148 (3) , pp. 473–86, 2012.
    • G. Junion and K. Jagla, “ChIP-enriched in silico targets (ChEST), a ChIP-on-chip approach applied to analyzing skeletal muscle genes.”, Meth. Mol. Biol., vol. 798 , pp. 543–53, 2012.
    • C. de Joussineau, L. Bataille, T. Jagla and K. Jagla, “Diversification of muscle types in Drosophila: upstream and downstream of identity genes.”, Curr. Top. Dev. Biol., vol. 98 , pp. 277–301, 2012.
  • 2011
  • 2010
    • L. Bataille, I. Delon, J. Da Ponte, N. Brown and K. Jagla, “Downstream of identity genes: muscle-type-specific regulation of the fusion process.”, Dev. Cell, vol. 19 (2) , pp. 317–28, 2010.
    • V. Tixier, L. Bataille and K. Jagla, “Diversification of muscle types: recent insights from Drosophila.”, Exp. Cell Res., vol. 316 (18) , pp. 3019–27, 2010.
    • N. Figeac, T. Jagla, R. Aradhya, J. Da Ponte and K. Jagla, “Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway.”, Development, vol. 137 (12) , pp. 1965–73, 2010.
    • M. Daczewska, L. Picchio, T. Jagla, N. Figeac and K. Jagla, “Muscle development and regeneration in normal and pathological conditions: learning from Drosophila.”, Curr. Pharm. Des., vol. 16 (8) , pp. 929–41, 2010.
    • D. Muller, T. Jagla, L. Bodart, N. Jahrling, H. Dodt, K. Jagla and M. Frasch, “Regulation and functions of the lms homeobox gene during development of embryonic lateral transverse muscles and direct flight muscles in Drosophila.”, PLoS ONE, vol. 5 (12) , pp. e14323, 2010.
  • 2009
    • H. Meyer, M. Panz, M. Zmojdzian, K. Jagla and A. Paululat, “Neprilysin 4, a novel endopeptidase from Drosophila melanogaster, displays distinct substrate specificities and exceptional solubility states.”, J. Exp. Biol., vol. 212 (Pt 22) , pp. 3673–83, 2009.
    • A. Kacperczyk, T. Jagla and M. Daczewska, “Pax-3 and Pax-7 label muscle progenitor cells during myotomal myogenesis in Coregonus lavaretus (Teleostei: Coregonidae).”, Anat Histol Embryol, vol. 38 (6) , pp. 411–8, 2009.
  • 2008
    • C. Medioni, M. Astier, M. Zmojdzian, K. Jagla and M. Semeriva, “Genetic control of cell morphogenesis during Drosophila melanogaster cardiac tube formation.”, J. Cell Biol., vol. 182 (2) , pp. 249–61, 2008.
    • M. Zmojdzian, J. Da Ponte and K. Jagla, “Cellular components and signals required for the cardiac outflow tract assembly in Drosophila.”, Proc. Natl. Acad. Sci. U.S.A., vol. 105 (7) , pp. 2475–80, 2008.