Team 10: Translational approach to epithelial injury and repair

Retinoids (active derivatives of vitamin A) are fundamental morphogens for the harmonious development of mammals.  We previously initiated the study of the implications of retinoids during the normal and pathological development of two human tissues (amniotic membranes and the lung) by establishing the functionality of the metabolic and molecular cascades of retinoids.


The purpose of our project is to identify new tissue, cellular, and molecular sequences regulated by retinoids. The dysregulation of these sequences could be one of the pathophysiological mechanisms that explain the abnormal development of the lung and the amniotic membranes. A definition of the retinoid target genes is established for this purpose using a transcriptomic approach combined with bioinformatic analysis. The integration of target genes in large physiological cascades will provide a more complete picture of the involvement of retinoids in these two tissues. An epigenetic approach will complement this approach. In the long term, the data obtained will make it possible to improve the diagnosis and treatment of lung development disorders (diaphragmatic congenital hernia) and amniotic membrane pathologies (rupture of the membranes).

Research thematics


Last Name First Name Position Contact
Floriane AUZANCE profile picture AUZANCE Floriane Intern
Corinne BELVILLE profile picture BELVILLE Corinne Research Engineer
Loic BLANCHON profile picture BLANCHON Loic Associate Professor
Raiko BLONDONNET profile picture BLONDONNET Raiko Ph.D Student
Damien BOUVIER profile picture BOUVIER Damien Clinician
Martin CHARVIN profile picture CHARVIN Martin Intern
Frederic CHIAMBARETTA profile picture CHIAMBARETTA Frederic Professor - Clinician
Helena CHOLTUS profile picture CHOLTUS Helena Intern
Jean-Michel CONSTANTIN profile picture CONSTANTIN Jean-Michel Professor - Clinician
Karen COSTE profile picture COSTE Karen Associate Professor - Clinician
Emmanuel FUTIER profile picture FUTIER Emmanuel Professor - Clinician
Denis GALLOT profile picture GALLOT Denis Professor - Clinician
Christelle GROSS profile picture GROSS Christelle Ph.D Student
Matthieu JABAUDON profile picture JABAUDON Matthieu Associate Professor - Clinician
Marilyne LAVERGNE profile picture LAVERGNE Marilyne Ph.D Student
Geoffroy MARCEAU profile picture MARCEAU Geoffroy Associate Professor - Clinician
Regine MINET-QUINARD profile picture MINET-QUINARD Regine Associate Professor - Clinician
Sebastien PERBET profile picture PERBET Sebastien Ph.D Student
Laurence ROSZYK profile picture ROSZYK Laurence Clinician
Vincent SAPIN profile picture SAPIN Vincent Principal Investigator


  • 2017
    • C. Huret, B. Pereira, V. Collange, A. Delabaere, M. Rouzaire, D. Lemery, V. Sapin and D. Gallot, “[Premature rupture of membranes>/=37 weeks of gestation: Predictive factors for labour onset within 24hours].”, Gynecologie, obstetrique, fertilite & senologie, vol. 45 (6) , pp. 348–352, 2017.
    • S. Bulyez, B. Pereira, E. Caumon, E. Imhoff, L. Roszyk, L. Bernard, L. Buhler, C. Heidegger, S. Jaber, J. Lefrant, R. Chabanne, P. Bertrand, P. Laterre, P. Guerci, P. Danin, E. Escudier, A. Sossou, D. Morand, V. Sapin, J. Constantin and M. Jabaudon, “Epidural analgesia in critically ill patients with acute pancreatitis: the multicentre randomised controlled EPIPAN study protocol.”, BMJ open, vol. 7 (5) , pp. e015280, 2017.
    • D. Bouvier, T. Duret, M. Abbot, T. Stiernon, B. Pereira, A. Coste, J. Chazal and V. Sapin, “Utility of S100B Serum Level for the Determination of Concussion in Male Rugby Players.”, Sports medicine (Auckland, N.Z.), vol. 47 (4) , pp. 781–789, 2017.
    • M. Jabaudon, P. Boucher, E. Imhoff, R. Chabanne, J. Faure, L. Roszyk, S. Thibault, R. Blondonnet, G. Clairefond, R. Guerin, S. Perbet, S. Cayot, T. Godet, B. Pereira, V. Sapin, J. Bazin, E. Futier and J. Constantin, “Sevoflurane for Sedation in Acute Respiratory Distress Syndrome. A Randomized Controlled Pilot Study.”, American journal of respiratory and critical care medicine, vol. 195 (6) , pp. 792–800, 2017.
    • M. Jabaudon, T. Godet, E. Futier, J. Bazin, V. Sapin, L. Roszyk, B. Pereira and J. Constantin, “Rationale, study design and analysis plan of the lung imaging morphology for ventilator settings in acute respiratory distress syndrome study (LIVE study): Study protocol for a randomised controlled trial.”, Anaesthesia, critical care & pain medicine, 2017.
    • G. Bellani, J. Rouby, J. Constantin and A. Pesenti, “Looking closer at acute respiratory distress syndrome: the role of advanced imaging techniques.”, Current opinion in critical care, vol. 23 (1) , pp. 30–37, 2017.
    • A. Delabaere, G. Marceau, K. Coste, L. Blanchon, P. Dechelotte, P. Blanc, V. Sapin and D. Gallot, “Effects of tracheal occlusion with retinoic acid administration on normal lung development.”, Prenatal diagnosis, 2017.
    • C. Prat, C. Belville, A. Comptour, G. Marceau, G. Clairefond, F. Chiambaretta, V. Sapin and L. Blanchon, “Myocilin expression is regulated by retinoic acid in the trabecular meshwork-derived cellular environment.”, Experimental eye research, vol. 155 , pp. 91–98, 2017.
  • 2016
    • M. Rouzaire, A. Comptour, C. Belville, D. Bouvier, G. Clairefond, F. Ponelle, V. Sapin, D. Gallot and L. Blanchon, “All-trans retinoic acid promotes wound healing of primary amniocytes through the induction of LOXL4, a member of the lysyl oxidase family.”, The international journal of biochemistry & cell biology, vol. 81 (Pt A) , pp. 10–19, 2016.
    • S. Mrozek, M. Jabaudon, S. Jaber, C. Paugam-Burtz, J. Lefrant, J. Rouby, K. Asehnoune, B. Allaouchiche, O. Baldesi, M. Leone, Q. Lu, J. Bazin, L. Roszyk, V. Sapin, E. Futier, B. Pereira and J. Constantin, “Elevated Plasma Levels of sRAGE Are Associated With Nonfocal CT-Based Lung Imaging in Patients With ARDS: A Prospective Multicenter Study.”, Chest, vol. 150 (5) , pp. 998–1007, 2016.
    • M. Haddam, L. Zieleskiewicz, S. Perbet, A. Baldovini, C. Guervilly, C. Arbelot, A. Noel, C. Vigne, E. Hammad, F. Antonini, S. Lehingue, E. Peytel, Q. Lu, B. Bouhemad, J. Golmard, O. Langeron, C. Martin, L. Muller, J. Rouby, J. Constantin, L. Papazian and M. Leone, “Lung ultrasonography for assessment of oxygenation response to prone position ventilation in ARDS.”, Intensive care medicine, vol. 42 (10) , pp. 1546–56, 2016.
    • A. Comptour, M. Rouzaire, C. Belville, D. Bouvier, D. Gallot, L. Blanchon and V. Sapin, “Nuclear retinoid receptors and pregnancy: placental transfer, functions, and pharmacological aspects.”, Cell. Mol. Life Sci., vol. 73 (20) , pp. 3823–37, 2016.
    • A. Comptour, M. Rouzaire, C. Belville, N. Bonnin, E. Daniel, F. Chiambaretta, L. Blanchon and V. Sapin, “Lysyl oxidase-like 4 involvement in retinoic acid epithelial wound healing.”, Scientific reports, vol. 6 , pp. 32688, 2016.
    • C. Dionet, M. Muller-Barthelemy, G. Marceau, J. Denis, D. Averbeck, J. Gueulette, V. Sapin, B. Pereira, A. Tchirkov, E. Chautard and P. Verrelle, “Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons.”, International journal of radiation biology, vol. 92 (9) , pp. 527–35, 2016.
    • J. Frat, S. Ragot, C. Girault, S. Perbet, G. Prat, T. Boulain, A. Demoule, J. Ricard, R. Coudroy, R. Robert, A. Mercat, L. Brochard and A. Thille, “Effect of non-invasive oxygenation strategies in immunocompromised patients with severe acute respiratory failure: a post-hoc analysis of a randomised trial.”, The Lancet. Respiratory medicine, vol. 4 (8) , pp. 646–52, 2016.
    • D. Bouvier, T. Duret, P. Rouzaire, M. Jabaudon, M. Rouzaire, C. Nourrisson, C. Bourgne, B. Pereira, B. Evrard and V. Sapin, “Preanalytical, analytical, gestational and pediatric aspects of the S100B immuno-assays.”, Clinical chemistry and laboratory medicine, vol. 54 (5) , pp. 833–42, 2016.
    • S. Jaber, T. Lescot, E. Futier, C. Paugam-Burtz, P. Seguin, M. Ferrandiere, S. Lasocki, O. Mimoz, B. Hengy, A. Sannini, J. Pottecher, P. Abback, B. Riu, F. Belafia, J. Constantin, E. Masseret, M. Beaussier, D. Verzilli, A. De Jong, G. Chanques, L. Brochard and N. Molinari, “Effect of Noninvasive Ventilation on Tracheal Reintubation Among Patients With Hypoxemic Respiratory Failure Following Abdominal Surgery: A Randomized Clinical Trial.”, JAMA, vol. 315 (13) , pp. 1345–53, 2016.
    • M. Jabaudon, R. Blondonnet, J. Lutz, L. Roszyk, D. Bouvier, R. Guerin, S. Perbet, S. Cayot, T. Godet, L. Blanchon, J. Bazin, E. Futier, V. Sapin and J. Constantin, “Net alveolar fluid clearance is associated with lung morphology phenotypes in acute respiratory distress syndrome.”, Anaesthesia, critical care & pain medicine, vol. 35 (2) , pp. 81–6, 2016.
    • R. Blondonnet, J. Constantin, V. Sapin and M. Jabaudon, “A Pathophysiologic Approach to Biomarkers in Acute Respiratory Distress Syndrome.”, Disease markers, vol. 2016 , pp. 3501373, 2016.
  • 2015
    • M. Jabaudon, E. Futier, L. Roszyk, V. Sapin, B. Pereira and J. Constantin, “Association between intraoperative ventilator settings and plasma levels of soluble receptor for advanced glycation end-products in patients without pre-existing lung injury.”, Respirology (Carlton, Vic.), vol. 20 (7) , pp. 1131–8, 2015.
    • D. Bouvier, M. Rouzaire, G. Marceau, C. Prat, B. Pereira, R. Lemarie, P. Deruelle, I. Fajardy, D. Gallot, L. Blanchon, A. Vambergue and V. Sapin, “Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin.”, J. Clin. Endocrinol. Metab., vol. 100 (10) , pp. E1270–9, 2015.
    • C. Prat, D. Bouvier, A. Comptour, G. Marceau, C. Belville, G. Clairefond, P. Blanc, D. Gallot, L. Blanchon and V. Sapin, “All-trans-retinoic acid regulates aquaporin-3 expression and related cellular membrane permeability in the human amniotic environment.”, Placenta, vol. 36 (8) , pp. 881–7, 2015.
    • M. Jabaudon, R. Blondonnet, L. Roszyk, D. Bouvier, J. Audard, G. Clairefond, M. Fournier, G. Marceau, P. Dechelotte, B. Pereira, V. Sapin and J. Constantin, “Soluble Receptor for Advanced Glycation End-Products Predicts Impaired Alveolar Fluid Clearance in Acute Respiratory Distress Syndrome.”, American journal of respiratory and critical care medicine, vol. 192 (2) , pp. 191–9, 2015.
    • D. Gallot, F. Goffinet, V. Sapin, J. Guibourdenche, M. Jouannic, B. Langer, M. Doret, D. Subtil and H. Fernandez, “Comment and reply on: Guidelines for the management of spontaneous preterm labor: identification of spontaneous preterm labor, diagnosis of preterm premature rupture of membranes, and preventive tools for preterm birth.”, The journal of maternal-fetal & neonatal medicine, pp. 1–2, 2015.
    • J. Frat, A. Thille, A. Mercat, C. Girault, S. Ragot, S. Perbet, G. Prat, T. Boulain, E. Morawiec, A. Cottereau, J. Devaquet, S. Nseir, K. Razazi, J. Mira, L. Argaud, J. Chakarian, J. Ricard, X. Wittebole, S. Chevalier, A. Herbland, M. Fartoukh, J. Constantin, J. Tonnelier, M. Pierrot, A. Mathonnet, G. Beduneau, C. Deletage-Metreau, J. Richard, L. Brochard and R. Robert, “High-flow oxygen through nasal cannula in acute hypoxemic respiratory failure.”, The New England journal of medicine, vol. 372 (23) , pp. 2185–96, 2015.
    • M. Jabaudon, N. Hamroun, L. Roszyk, R. Guerin, J. Bazin, V. Sapin, B. Pereira and J. Constantin, “Effects of a recruitment maneuver on plasma levels of soluble RAGE in patients with diffuse acute respiratory distress syndrome: a prospective randomized crossover study.”, Intensive care medicine, vol. 41 (5) , pp. 846–55, 2015.
    • R. Blondonnet, J. Aliane, T. Godet, B. Souweine and J. Constantin, “High-frequency percussive ventilation as a rescue therapy for ARDS patients under ECMO: About a case.”, Anaesthesia, critical care & pain medicine, vol. 34 (2) , pp. 105–7, 2015.
    • S. Barbaux, J. Erwich, P. Favaron, S. Gil, D. Gallot, T. Golos, A. Gonzalez-Bulnes, J. Guibourdenche, A. Heazell, T. Jansson, O. Laprevote, R. Lewis, R. Miller, D. Monk, B. Novakovic, C. Oudejans, M. Parast, P. Peugnet, C. Pfarrer, H. Pinar, C. Roberts, W. Robinson, R. Saffery, C. Salomon, A. Sexton, A. Staff, M. Suter, A. Tarrade, J. Wallace, C. Vaillancourt, D. Vaiman, S. Worton and G. Lash, “IFPA meeting 2014 workshop report: Animal models to study pregnancy pathologies; new approaches to study human placental exposure to xenobiotics; biomarkers of pregnancy pathologies; placental genetics and epigenetics; the placenta and stillbirth and fetal growth restriction.”, Placenta, vol. 36 Suppl 1 , pp. S5–10, 2015.
    • K. Coste, L. Beurskens, P. Blanc, D. Gallot, A. Delabaere, L. Blanchon, D. Tibboel, A. Labbe, R. Rottier and V. Sapin, “Metabolic disturbances of the vitamin A pathway in human diaphragmatic hernia.”, American journal of physiology. Lung cellular and molecular physiology, vol. 308 (2) , pp. L147–57, 2015.
    • M. Jabaudon, R. Blondonnet, L. Roszyk, B. Pereira, R. Guerin, S. Perbet, S. Cayot, D. Bouvier, L. Blanchon, V. Sapin and J. Constantin, “Soluble Forms and Ligands of the Receptor for Advanced Glycation End-Products in Patients with Acute Respiratory Distress Syndrome: An Observational Prospective Study.”, PLoS ONE, vol. 10 (8) , pp. e0135857, 2015.
  • 2014
    • D. Menshykau, P. Blanc, E. Unal, V. Sapin and D. Iber, “An interplay of geometry and signaling enables robust lung branching morphogenesis.”, Development, vol. 141 (23) , pp. 4526–36, 2014.
    • A. Rossi, A. Delabaere, H. Delmas-Laurichesse, A. Beaufrere, D. Lemery and D. Gallot, “The challenge of prenatal identification of congenital diaphragmatic hernia in the context of hydrops.”, European journal of obstetrics, gynecology, and reproductive biology, vol. 182 , pp. 238–9, 2014.
    • R. Chabanne, S. Perbet, E. Futier, N. Ben Said, S. Jaber, J. Bazin, B. Pereira and J. Constantin, “Impact of the anesthetic conserving device on respiratory parameters and work of breathing in critically ill patients under light sedation with sevoflurane.”, Anesthesiology, vol. 121 (4) , pp. 808–16, 2014.
    • C. Dunand, P. Hoffmann, V. Sapin, L. Blanchon, A. Salomon, F. Sergent, M. Benharouga, S. Sabra, J. Guibourdenche, S. Lye, J. Feige and N. Alfaidy, “Endocrine gland-derived endothelial growth factor (EG-VEGF) is a potential novel regulator of human parturition.”, Biol Reprod, vol. 91 (3) , pp. 73, 2014.
    • N. Bonnin, C. Belville, F. Chiambaretta, V. Sapin and L. Blanchon, “DNA methyl transferases are differentially expressed in the human anterior eye segment.”, Acta ophthalmologica, vol. 92 (5) , pp. e366–71, 2014.
    • R. Deplus, L. Blanchon, A. Rajavelu, A. Boukaba, M. Defrance, J. Luciani, F. Rothe, S. Dedeurwaerder, H. Denis, A. Brinkman, F. Simmer, F. Muller, B. Bertin, M. Berdasco, P. Putmans, E. Calonne, D. Litchfield, Y. de Launoit, T. Jurkowski, H. Stunnenberg, C. Bock, C. Sotiriou, M. Fraga, M. Esteller, A. Jeltsch and F. Fuks, “Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a.”, Cell Rep, vol. 8 (3) , pp. 743–53, 2014.
    • F. Dutheil, G. Walther, R. Chapier, G. Mnatzaganian, B. Lesourd, G. Naughton, J. Verney, A. Fogli, V. Sapin, M. Duclos, A. Vinet, P. Obert, D. Courteix and G. Lac, “Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet - the RESOLVE trial.”, Lipids in health and disease, vol. 13 , pp. 112, 2014.
    • A. Ravinet, S. Perbet, R. Guieze, R. Lemal, R. Guerin, G. Gayraud, J. Aliane, A. Tremblay, J. Pascal, A. Ledoux, C. Chaleteix, P. Dechelotte, J. Bay, J. Bazin and J. Constantin, “Lung postmortem autopsy revealing extramedullary involvement in multiple myeloma causing acute respiratory distress syndrome.”, Case reports in hematology, vol. 2014 , pp. 635237, 2014.
  • 2013
    • JM. Lobaccaro, D. Gallot, S. Lumbroso and K. Mouzat, “Liver X Receptors and female reproduction: when cholesterol meets fertility!”, Journal of endocrinological investigation, vol. 36 (1) , pp. 55–60, 2013.
    • M. Jabaudon, S. Perbet, B. Pereira, A. Soummer, L. Roszyk, R. Guerin, E. Futier, Q. Lu, J. Bazin, V. Sapin, J. Rouby and J. Constantin, “Plasma levels of sRAGE, loss of aeration and weaning failure in ICU patients: a prospective observational multicenter study.”, PLoS ONE, vol. 8 (5) , pp. e64083, 2013.
  • 2012
  • 2010
    • A. Albert, M. Gaume, S. Ughetto, V. Sapin and A. Fogli, “[Coupling proteinemia and serum protein electrophoresis: evaluation of the capillary technique (Capillarys 2, Sebia), experience from Clermont-Ferrand].”, Ann. Biol. Clin. (Paris), vol. 68 (6) , pp. 657–67, 2010.
    • I. Sahut-Barnola, C. de Joussineau, P. Val, S. Lambert-Langlais, C. Damon, A. Lefrancois-Martinez, J. Pointud, G. Marceau, V. Sapin, F. Tissier, B. Ragazzon, J. Bertherat, L. Kirschner, C. Stratakis and A. Martinez, “Cushing's syndrome and fetal features resurgence in adrenal cortex-specific Prkar1a knockout mice.”, PLoS Genet., vol. 6 (6) , pp. e1000980, 2010.
    • J. Talvas, C. Caris-Veyrat, L. Guy, M. Rambeau, B. Lyan, R. Minet-Quinard, JM. Lobaccaro, M. Vasson, S. George, A. Mazur and E. Rock, “Differential effects of lycopene consumed in tomato paste and lycopene in the form of a purified extract on target genes of cancer prostatic cells.”, The American journal of clinical nutrition, vol. 91 (6) , pp. 1716–24, 2010.
    • C. Goumy, L. Gouas, G. Marceau, K. Coste, L. Veronese, D. Gallot, V. Sapin, P. Vago and A. Tchirkov, “Retinoid pathway and congenital diaphragmatic hernia: hypothesis from the analysis of chromosomal abnormalities.”, Fetal. Diagn. Ther., vol. 28 (3) , pp. 129–39, 2010.
    • C. Goumy, K. Coste, G. Marceau, L. Gouas, A. Tchirkov, P. Vago, D. Gallot and V. Sapin, “Fetal skin fibroblasts: a cell model for studying the retinoid pathway in congenital diaphragmatic hernia.”, Birth Defects Res. Part A Clin. Mol. Teratol., vol. 88 (3) , pp. 195–200, 2010.
  • 2009
    • I. Sahut-Barnola, C. De Joussineau, P. Val, S. Lambert-Langlais, A. Lefrancois-Martinez, J. Pointud, G. Marceau, V. Sapin, B. Ragazzon, J. Bertherat, L. Kirschner, C. Stratakis and A. Martinez, “Mouse model for bilateral adrenal hyperplasia.”, Annales d'endocrinologie, vol. 70 (3) , pp. 194, 2009.
    • C. Belville, J. Marechal, S. Pennetier, P. Carmillo, L. Masgrau, L. Messika-Zeitoun, J. Galey, G. Machado, D. Treton, J. Gonzales, J. Picard, N. Josso, R. Cate and N. di Clemente, “Natural mutations of the anti-Mullerian hormone type II receptor found in persistent Mullerian duct syndrome affect ligand binding, signal transduction and cellular transport.”, Hum. Mol. Genet., vol. 18 (16) , pp. 3002–13, 2009.
  • 2008