Team 10: Translational approach to epithelial injury and repair

Our research team is interested in the membrane receptor called RAGE (Receptor for Advanced glycation Products) which is a multi-ligands (called alarmins) receptor belonging to the immunoglobulin superfamily. Ligands-binding activate, at the cellular level, signalling pathways leading to an inflammatory answer. Our project is particularly focused on this receptor implication in lung and fetal membranes epithelium aggression during two pathologies called respectively: Acute Respiratory Distress Syndrome (ARDS) and Premature Rupture of Membrane (PROM).

Research

Following endogenous or exogenous aggression, the integrity and functionality of the epithelial barrier could be disrupted as encountered in numerous pathologies. Wound healing ability after such aggression is of primary importance in order to obtain a clinical positive evolution. Situated on the crossroads between metabolic and inflammatory cellular process, the Receptor for Advanced glycation Products (RAGE) seems to be a promising research area in such epithelial defects. The team has already started to demonstrate RAGE’ importance in lung and fetal membranes epithelial aggression models encountered in two pathologies called Acute Respiratory Distress Syndrome (ARDS) and Premature Rupture of Membrane (PROM).  Due to the numerous potential associations between RAGE and its ligands directly linked to pathological state, the importance of each combination and/or the research of new RAGE ligands had to be clarified and identified in ARDS or PROM. By using pharmacological approach and molecular biology tools, our research project aims to identify molecular pathways and target genes that could be implied in pathophysiological cases regarding both pathologies. Furthermore, as the direct interaction of the epithelium with its surrounding environment constitute an important part of the wound evolution; our work will also demonstrate the RAGE pathway implications regarding cell communications. The availability of a RAGE KO mouse in the lab, will permit us to conduct in-vivo studies. At the end, all our results must permit the arising of new diagnostics and therapeutics tools in our both pathologies.

Research thematics

People

Last Name First Name Position Contact
Corinne BELVILLE profile picture BELVILLE Corinne Research Engineer
Loic BLANCHON profile picture BLANCHON Loic Associate Professor
Raiko BLONDONNET profile picture BLONDONNET Raiko Ph.D Student
Damien BOUVIER profile picture BOUVIER Damien Clinician
Frederic CHIAMBARETTA profile picture CHIAMBARETTA Frederic Professor - Clinician
Jean-Michel CONSTANTIN profile picture CONSTANTIN Jean-Michel Professor - Clinician
Karen COSTE profile picture COSTE Karen Associate Professor - Clinician
Emmanuel FUTIER profile picture FUTIER Emmanuel Professor - Clinician
Denis GALLOT profile picture GALLOT Denis Professor - Clinician
Christelle GROSS profile picture GROSS Christelle Ph.D Student
Matthieu JABAUDON profile picture JABAUDON Matthieu Associate Professor - Clinician
Marilyne LAVERGNE profile picture LAVERGNE Marilyne Ph.D Student
Geoffroy MARCEAU profile picture MARCEAU Geoffroy Associate Professor - Clinician
Regine MINET-QUINARD profile picture MINET-QUINARD Regine Associate Professor - Clinician
Bertille PAQUETTE profile picture PAQUETTE Bertille Intern
Sebastien PERBET profile picture PERBET Sebastien Ph.D Student
Laurence ROSZYK profile picture ROSZYK Laurence Clinician
Vincent SAPIN profile picture SAPIN Vincent Principal Investigator
Marine SERVEAUX DANCER profile picture SERVEAUX DANCER Marine Assistant Clinician

Publications

  • 2018
    • M. Jabaudon, R. Blondonnet, J. Audard, M. Fournet, T. Godet, V. Sapin and J. Constantin, “Recent directions in personalised acute respiratory distress syndrome medicine.”, Anaesthesia, critical care & pain medicine, vol. 37 (3) , pp. 251–258, 2018.
    • C. Oris, B. Pereira, J. Durif, J. Simon-Pimmel, C. Castellani, S. Manzano, V. Sapin and D. Bouvier, “The Biomarker S100B and Mild Traumatic Brain Injury: A Meta-analysis.”, Pediatrics, 2018.
    • A. Delabaere, L. Blanchon, K. Coste, G. Clairefond, C. Belville, P. Blanc, G. Marceau, V. Sapin and D. Gallot, “Retinoic acid and tracheal occlusion for diaphragmatic hernia treatment in rabbit fetuses.”, Prenatal diagnosis, 2018.
    • M. Jabaudon, P. Berthelin, T. Pranal, L. Roszyk, T. Godet, J. Faure, R. Chabanne, N. Eisenmann, A. Lautrette, C. Belville, R. Blondonnet, S. Cayot, T. Gillart, J. Pascal, Y. Skrzypczak, B. Souweine, L. Blanchon, V. Sapin, B. Pereira and J. Constantin, “Receptor for advanced glycation end-products and ARDS prediction: a multicentre observational study.”, Scientific reports, vol. 8 (1) , pp. 2603, 2018.
    • T. Godet, M. Jabaudon, R. Blondonnet, A. Tremblay, J. Audard, B. Rieu, B. Pereira, J. Garcier, E. Futier and J. Constantin, “High frequency percussive ventilation increases alveolar recruitment in early acute respiratory distress syndrome: an experimental, physiological and CT scan study.”, Critical care (London, England), vol. 22 (1) , pp. 3, 2018.
    • I. Soudy, R. Minet-Quinard, A. Mahamat, H. Ngoua, A. Izzedine, A. Tidjani, E. Ngo Bum, C. Lambert, B. Pereira, J. Desjeux and V. Sapin, “Vitamin A status in healthy women eating traditionally prepared spirulina (Dihe) in the Chad Lake area.”, PLoS ONE, vol. 13 (1) , pp. e0191887, 2018.
    • C. Cheung, D. Anderson, M. Rouzaire, L. Blanchon, V. Sapin and R. Brace, “Retinoic Acid Pathway Regulation of Vascular Endothelial Growth Factor in Ovine Amnion.”, Reproductive sciences (Thousand Oaks, Calif.), pp. 1933719118765979, 2018.
  • 2017
  • 2016
    • M. Rouzaire, A. Comptour, C. Belville, D. Bouvier, G. Clairefond, F. Ponelle, V. Sapin, D. Gallot and L. Blanchon, “All-trans retinoic acid promotes wound healing of primary amniocytes through the induction of LOXL4, a member of the lysyl oxidase family.”, The international journal of biochemistry & cell biology, vol. 81 (Pt A) , pp. 10–19, 2016.
    • S. Mrozek, M. Jabaudon, S. Jaber, C. Paugam-Burtz, J. Lefrant, J. Rouby, K. Asehnoune, B. Allaouchiche, O. Baldesi, M. Leone, Q. Lu, J. Bazin, L. Roszyk, V. Sapin, E. Futier, B. Pereira and J. Constantin, “Elevated Plasma Levels of sRAGE Are Associated With Nonfocal CT-Based Lung Imaging in Patients With ARDS: A Prospective Multicenter Study.”, Chest, vol. 150 (5) , pp. 998–1007, 2016.
    • M. Haddam, L. Zieleskiewicz, S. Perbet, A. Baldovini, C. Guervilly, C. Arbelot, A. Noel, C. Vigne, E. Hammad, F. Antonini, S. Lehingue, E. Peytel, Q. Lu, B. Bouhemad, J. Golmard, O. Langeron, C. Martin, L. Muller, J. Rouby, J. Constantin, L. Papazian and M. Leone, “Lung ultrasonography for assessment of oxygenation response to prone position ventilation in ARDS.”, Intensive care medicine, vol. 42 (10) , pp. 1546–56, 2016.
    • A. Comptour, M. Rouzaire, C. Belville, D. Bouvier, D. Gallot, L. Blanchon and V. Sapin, “Nuclear retinoid receptors and pregnancy: placental transfer, functions, and pharmacological aspects.”, Cell. Mol. Life Sci., vol. 73 (20) , pp. 3823–37, 2016.
    • A. Comptour, M. Rouzaire, C. Belville, N. Bonnin, E. Daniel, F. Chiambaretta, L. Blanchon and V. Sapin, “Lysyl oxidase-like 4 involvement in retinoic acid epithelial wound healing.”, Scientific reports, vol. 6 , pp. 32688, 2016.
    • C. Dionet, M. Muller-Barthelemy, G. Marceau, J. Denis, D. Averbeck, J. Gueulette, V. Sapin, B. Pereira, A. Tchirkov, E. Chautard and P. Verrelle, “Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons.”, International journal of radiation biology, vol. 92 (9) , pp. 527–35, 2016.
    • J. Frat, S. Ragot, C. Girault, S. Perbet, G. Prat, T. Boulain, A. Demoule, J. Ricard, R. Coudroy, R. Robert, A. Mercat, L. Brochard and A. Thille, “Effect of non-invasive oxygenation strategies in immunocompromised patients with severe acute respiratory failure: a post-hoc analysis of a randomised trial.”, The Lancet. Respiratory medicine, vol. 4 (8) , pp. 646–52, 2016.
    • D. Bouvier, T. Duret, P. Rouzaire, M. Jabaudon, M. Rouzaire, C. Nourrisson, C. Bourgne, B. Pereira, B. Evrard and V. Sapin, “Preanalytical, analytical, gestational and pediatric aspects of the S100B immuno-assays.”, Clinical chemistry and laboratory medicine, vol. 54 (5) , pp. 833–42, 2016.
    • S. Jaber, T. Lescot, E. Futier, C. Paugam-Burtz, P. Seguin, M. Ferrandiere, S. Lasocki, O. Mimoz, B. Hengy, A. Sannini, J. Pottecher, P. Abback, B. Riu, F. Belafia, J. Constantin, E. Masseret, M. Beaussier, D. Verzilli, A. De Jong, G. Chanques, L. Brochard and N. Molinari, “Effect of Noninvasive Ventilation on Tracheal Reintubation Among Patients With Hypoxemic Respiratory Failure Following Abdominal Surgery: A Randomized Clinical Trial.”, JAMA, vol. 315 (13) , pp. 1345–53, 2016.
    • M. Jabaudon, R. Blondonnet, J. Lutz, L. Roszyk, D. Bouvier, R. Guerin, S. Perbet, S. Cayot, T. Godet, L. Blanchon, J. Bazin, E. Futier, V. Sapin and J. Constantin, “Net alveolar fluid clearance is associated with lung morphology phenotypes in acute respiratory distress syndrome.”, Anaesthesia, critical care & pain medicine, vol. 35 (2) , pp. 81–6, 2016.
    • R. Blondonnet, J. Constantin, V. Sapin and M. Jabaudon, “A Pathophysiologic Approach to Biomarkers in Acute Respiratory Distress Syndrome.”, Disease markers, vol. 2016 , pp. 3501373, 2016.
  • 2015
    • M. Jabaudon, E. Futier, L. Roszyk, V. Sapin, B. Pereira and J. Constantin, “Association between intraoperative ventilator settings and plasma levels of soluble receptor for advanced glycation end-products in patients without pre-existing lung injury.”, Respirology (Carlton, Vic.), vol. 20 (7) , pp. 1131–8, 2015.
    • D. Bouvier, M. Rouzaire, G. Marceau, C. Prat, B. Pereira, R. Lemarie, P. Deruelle, I. Fajardy, D. Gallot, L. Blanchon, A. Vambergue and V. Sapin, “Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin.”, J. Clin. Endocrinol. Metab., vol. 100 (10) , pp. E1270–9, 2015.
    • C. Prat, D. Bouvier, A. Comptour, G. Marceau, C. Belville, G. Clairefond, P. Blanc, D. Gallot, L. Blanchon and V. Sapin, “All-trans-retinoic acid regulates aquaporin-3 expression and related cellular membrane permeability in the human amniotic environment.”, Placenta, vol. 36 (8) , pp. 881–7, 2015.
    • M. Jabaudon, R. Blondonnet, L. Roszyk, D. Bouvier, J. Audard, G. Clairefond, M. Fournier, G. Marceau, P. Dechelotte, B. Pereira, V. Sapin and J. Constantin, “Soluble Receptor for Advanced Glycation End-Products Predicts Impaired Alveolar Fluid Clearance in Acute Respiratory Distress Syndrome.”, American journal of respiratory and critical care medicine, vol. 192 (2) , pp. 191–9, 2015.
    • D. Gallot, F. Goffinet, V. Sapin, J. Guibourdenche, M. Jouannic, B. Langer, M. Doret, D. Subtil and H. Fernandez, “Comment and reply on: Guidelines for the management of spontaneous preterm labor: identification of spontaneous preterm labor, diagnosis of preterm premature rupture of membranes, and preventive tools for preterm birth.”, The journal of maternal-fetal & neonatal medicine, pp. 1–2, 2015.
    • J. Frat, A. Thille, A. Mercat, C. Girault, S. Ragot, S. Perbet, G. Prat, T. Boulain, E. Morawiec, A. Cottereau, J. Devaquet, S. Nseir, K. Razazi, J. Mira, L. Argaud, J. Chakarian, J. Ricard, X. Wittebole, S. Chevalier, A. Herbland, M. Fartoukh, J. Constantin, J. Tonnelier, M. Pierrot, A. Mathonnet, G. Beduneau, C. Deletage-Metreau, J. Richard, L. Brochard and R. Robert, “High-flow oxygen through nasal cannula in acute hypoxemic respiratory failure.”, The New England journal of medicine, vol. 372 (23) , pp. 2185–96, 2015.
    • M. Jabaudon, N. Hamroun, L. Roszyk, R. Guerin, J. Bazin, V. Sapin, B. Pereira and J. Constantin, “Effects of a recruitment maneuver on plasma levels of soluble RAGE in patients with diffuse acute respiratory distress syndrome: a prospective randomized crossover study.”, Intensive care medicine, vol. 41 (5) , pp. 846–55, 2015.
    • R. Blondonnet, J. Aliane, T. Godet, B. Souweine and J. Constantin, “High-frequency percussive ventilation as a rescue therapy for ARDS patients under ECMO: About a case.”, Anaesthesia, critical care & pain medicine, vol. 34 (2) , pp. 105–7, 2015.
    • S. Barbaux, J. Erwich, P. Favaron, S. Gil, D. Gallot, T. Golos, A. Gonzalez-Bulnes, J. Guibourdenche, A. Heazell, T. Jansson, O. Laprevote, R. Lewis, R. Miller, D. Monk, B. Novakovic, C. Oudejans, M. Parast, P. Peugnet, C. Pfarrer, H. Pinar, C. Roberts, W. Robinson, R. Saffery, C. Salomon, A. Sexton, A. Staff, M. Suter, A. Tarrade, J. Wallace, C. Vaillancourt, D. Vaiman, S. Worton and G. Lash, “IFPA meeting 2014 workshop report: Animal models to study pregnancy pathologies; new approaches to study human placental exposure to xenobiotics; biomarkers of pregnancy pathologies; placental genetics and epigenetics; the placenta and stillbirth and fetal growth restriction.”, Placenta, vol. 36 Suppl 1 , pp. S5–10, 2015.
    • K. Coste, L. Beurskens, P. Blanc, D. Gallot, A. Delabaere, L. Blanchon, D. Tibboel, A. Labbe, R. Rottier and V. Sapin, “Metabolic disturbances of the vitamin A pathway in human diaphragmatic hernia.”, American journal of physiology. Lung cellular and molecular physiology, vol. 308 (2) , pp. L147–57, 2015.
    • M. Jabaudon, R. Blondonnet, L. Roszyk, B. Pereira, R. Guerin, S. Perbet, S. Cayot, D. Bouvier, L. Blanchon, V. Sapin and J. Constantin, “Soluble Forms and Ligands of the Receptor for Advanced Glycation End-Products in Patients with Acute Respiratory Distress Syndrome: An Observational Prospective Study.”, PLoS ONE, vol. 10 (8) , pp. e0135857, 2015.
  • 2014
    • D. Menshykau, P. Blanc, E. Unal, V. Sapin and D. Iber, “An interplay of geometry and signaling enables robust lung branching morphogenesis.”, Development, vol. 141 (23) , pp. 4526–36, 2014.
    • A. Rossi, A. Delabaere, H. Delmas-Laurichesse, A. Beaufrere, D. Lemery and D. Gallot, “The challenge of prenatal identification of congenital diaphragmatic hernia in the context of hydrops.”, European journal of obstetrics, gynecology, and reproductive biology, vol. 182 , pp. 238–9, 2014.
    • R. Chabanne, S. Perbet, E. Futier, N. Ben Said, S. Jaber, J. Bazin, B. Pereira and J. Constantin, “Impact of the anesthetic conserving device on respiratory parameters and work of breathing in critically ill patients under light sedation with sevoflurane.”, Anesthesiology, vol. 121 (4) , pp. 808–16, 2014.
    • C. Dunand, P. Hoffmann, V. Sapin, L. Blanchon, A. Salomon, F. Sergent, M. Benharouga, S. Sabra, J. Guibourdenche, S. Lye, J. Feige and N. Alfaidy, “Endocrine gland-derived endothelial growth factor (EG-VEGF) is a potential novel regulator of human parturition.”, Biol Reprod, vol. 91 (3) , pp. 73, 2014.
    • N. Bonnin, C. Belville, F. Chiambaretta, V. Sapin and L. Blanchon, “DNA methyl transferases are differentially expressed in the human anterior eye segment.”, Acta ophthalmologica, vol. 92 (5) , pp. e366–71, 2014.
    • R. Deplus, L. Blanchon, A. Rajavelu, A. Boukaba, M. Defrance, J. Luciani, F. Rothe, S. Dedeurwaerder, H. Denis, A. Brinkman, F. Simmer, F. Muller, B. Bertin, M. Berdasco, P. Putmans, E. Calonne, D. Litchfield, Y. de Launoit, T. Jurkowski, H. Stunnenberg, C. Bock, C. Sotiriou, M. Fraga, M. Esteller, A. Jeltsch and F. Fuks, “Regulation of DNA methylation patterns by CK2-mediated phosphorylation of Dnmt3a.”, Cell Rep, vol. 8 (3) , pp. 743–53, 2014.
    • F. Dutheil, G. Walther, R. Chapier, G. Mnatzaganian, B. Lesourd, G. Naughton, J. Verney, A. Fogli, V. Sapin, M. Duclos, A. Vinet, P. Obert, D. Courteix and G. Lac, “Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet - the RESOLVE trial.”, Lipids in health and disease, vol. 13 , pp. 112, 2014.
    • A. Ravinet, S. Perbet, R. Guieze, R. Lemal, R. Guerin, G. Gayraud, J. Aliane, A. Tremblay, J. Pascal, A. Ledoux, C. Chaleteix, P. Dechelotte, J. Bay, J. Bazin and J. Constantin, “Lung postmortem autopsy revealing extramedullary involvement in multiple myeloma causing acute respiratory distress syndrome.”, Case reports in hematology, vol. 2014 , pp. 635237, 2014.
  • 2013
    • JM. Lobaccaro, D. Gallot, S. Lumbroso and K. Mouzat, “Liver X Receptors and female reproduction: when cholesterol meets fertility!”, Journal of endocrinological investigation, vol. 36 (1) , pp. 55–60, 2013.
    • M. Jabaudon, S. Perbet, B. Pereira, A. Soummer, L. Roszyk, R. Guerin, E. Futier, Q. Lu, J. Bazin, V. Sapin, J. Rouby and J. Constantin, “Plasma levels of sRAGE, loss of aeration and weaning failure in ICU patients: a prospective observational multicenter study.”, PLoS ONE, vol. 8 (5) , pp. e64083, 2013.
  • 2012
  • 2010
    • A. Albert, M. Gaume, S. Ughetto, V. Sapin and A. Fogli, “[Coupling proteinemia and serum protein electrophoresis: evaluation of the capillary technique (Capillarys 2, Sebia), experience from Clermont-Ferrand].”, Ann. Biol. Clin. (Paris), vol. 68 (6) , pp. 657–67, 2010.
    • I. Sahut-Barnola, C. de Joussineau, P. Val, S. Lambert-Langlais, C. Damon, A. Lefrancois-Martinez, J. Pointud, G. Marceau, V. Sapin, F. Tissier, B. Ragazzon, J. Bertherat, L. Kirschner, C. Stratakis and A. Martinez, “Cushing's syndrome and fetal features resurgence in adrenal cortex-specific Prkar1a knockout mice.”, PLoS Genet., vol. 6 (6) , pp. e1000980, 2010.
    • J. Talvas, C. Caris-Veyrat, L. Guy, M. Rambeau, B. Lyan, R. Minet-Quinard, JM. Lobaccaro, M. Vasson, S. George, A. Mazur and E. Rock, “Differential effects of lycopene consumed in tomato paste and lycopene in the form of a purified extract on target genes of cancer prostatic cells.”, The American journal of clinical nutrition, vol. 91 (6) , pp. 1716–24, 2010.
    • C. Goumy, L. Gouas, G. Marceau, K. Coste, L. Veronese, D. Gallot, V. Sapin, P. Vago and A. Tchirkov, “Retinoid pathway and congenital diaphragmatic hernia: hypothesis from the analysis of chromosomal abnormalities.”, Fetal. Diagn. Ther., vol. 28 (3) , pp. 129–39, 2010.
    • C. Goumy, K. Coste, G. Marceau, L. Gouas, A. Tchirkov, P. Vago, D. Gallot and V. Sapin, “Fetal skin fibroblasts: a cell model for studying the retinoid pathway in congenital diaphragmatic hernia.”, Birth Defects Res. Part A Clin. Mol. Teratol., vol. 88 (3) , pp. 195–200, 2010.
  • 2009
    • I. Sahut-Barnola, C. De Joussineau, P. Val, S. Lambert-Langlais, A. Lefrancois-Martinez, J. Pointud, G. Marceau, V. Sapin, B. Ragazzon, J. Bertherat, L. Kirschner, C. Stratakis and A. Martinez, “Mouse model for bilateral adrenal hyperplasia.”, Annales d'endocrinologie, vol. 70 (3) , pp. 194, 2009.
    • C. Belville, J. Marechal, S. Pennetier, P. Carmillo, L. Masgrau, L. Messika-Zeitoun, J. Galey, G. Machado, D. Treton, J. Gonzales, J. Picard, N. Josso, R. Cate and N. di Clemente, “Natural mutations of the anti-Mullerian hormone type II receptor found in persistent Mullerian duct syndrome affect ligand binding, signal transduction and cellular transport.”, Hum. Mol. Genet., vol. 18 (16) , pp. 3002–13, 2009.
  • 2008