Claire CHAZAUD

Resume

Claire CHAZAUDprofile picture

Present: PI of the team: Genetic and epigenetic control of cell lineage commitment during mouse development.

2012: DR2 INSERM, PI at the GReD

2005 : CR1 INSERM, PI at the GReD

2003 to present: PI of team n°5 at the GReD (Clermont-Fd)

2002: Recipient of an "AVENIR" grant.

February 1999 to June 2003: post-doctoral fellow in Dr. Janet RossantLaboratory (Toronto, Canada). Research topics: 1- Cell lineage specification in the preimplantation mouse embryo. 2-Involvement of the Wnt/beta-Catenin pathway during A-P axis formation in the mouse.

1994-1998: PhD Université L. Pasteur,IGBMC (Strasbourg), director Dr. P. Dollé. Research topic: Characterisation of RA inducible genes expressed during  mouse development (supervisors Dr. P. Dollé and Prof. P. Chambon).

Research

We are looking for a Post-Doc!

Our team is interested in deciphering the molecular mechanisms regulating one of the first cell differentiation event that takes place in the embryo: the binary specification of epiblast (Epi) and Primitive Endoderm (PrE) within the Inner Cell Mass of the blastocyst during preimplantation.

We have recently described the role of Nanog (Frankenberg et al, 2011 Dev Cell) and Gata6 (Bessonnard et al, 2014 Development) and shown that the balance between these two transcription factors determines the number and the timing of Epi and PrE cell specification.

In collaboration with the team of A. Goldbeter/ G. Dupont a mathematical model was built (Bessonnard et al, 2014 Development) , uncovering novel features that were are currently investigating.

Publications

  • 2016
    • C. Chazaud and Y. Yamanaka, “Lineage specification in the mouse preimplantation embryo.”, Development, vol. 143 (7) , pp. 1063–74, 2016.
    • L. De Mot, D. Gonze, S. Bessonnard, C. Chazaud, A. Goldbeter and G. Dupont, “Cell Fate Specification Based on Tristability in the Inner Cell Mass of Mouse Blastocysts.”, Biophysical journal, vol. 110 (3) , pp. 710–22, 2016.
  • 2015
    • S. Chauveau and C. Chazaud, “Assessment of cell lineages and cell death in blastocysts by immunostaining.”, Meth. Mol. Biol., vol. 1222 , pp. 175–80, 2015.
  • 2014
    • S. Hermitte and C. Chazaud, “Primitive endoderm differentiation: from specification to epithelium formation.”, Philosophical transactions of the Royal Society of London. Series B, Biological sciences, vol. 369 (1657) , 2014.
    • S. Bessonnard, L. De Mot, D. Gonze, M. Barriol, C. Dennis, A. Goldbeter, G. Dupont and C. Chazaud, “Gata6, Nanog and Erk signaling control cell fate in the inner cell mass through a tristable regulatory network.”, Development, vol. 141 (19) , pp. 3637–48, 2014.
    • J. Artus and C. Chazaud, “A close look at the mammalian blastocyst: epiblast and primitive endoderm formation.”, Cell. Mol. Life Sci., vol. 71 (17) , pp. 3327–38, 2014.
  • 2013
  • 2012
    • F. Lavial, S. Bessonnard, Y. Ohnishi, A. Tsumura, A. Chandrashekran, M. Fenwick, R. Tomaz, H. Hosokawa, T. Nakayama, I. Chambers, T. Hiiragi, C. Chazaud and V. Azuara, “Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development.”, Genes Dev., vol. 26 (13) , pp. 1445–58, 2012.
  • 2011
    • S. Frankenberg, F. Gerbe, S. Bessonnard, C. Belville, P. Pouchin, O. Bardot and C. Chazaud, “Primitive endoderm differentiates via a three-step mechanism involving Nanog and RTK signaling.”, Dev. Cell, vol. 21 (6) , pp. 1005–13, 2011.
    • H. Elatmani, V. Dormoy-Raclet, P. Dubus, F. Dautry, C. Chazaud and H. Jacquemin-Sablon, “The RNA-binding protein Unr prevents mouse embryonic stem cells differentiation toward the primitive endoderm lineage.”, Stem cells (Dayton, Ohio), vol. 29 (10) , pp. 1504–16, 2011.
  • 2008
    • C. Chazaud, “[Early embryogenesis in mammals: stem cells and first commitment steps].”, Med Sci (Paris), vol. 24 (12) , pp. 1043–8, 2008.
    • F. Gerbe, B. Cox, J. Rossant and C. Chazaud, “Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst.”, Dev. Biol. (NY), vol. 313 (2) , pp. 594–602, 2008-01-15 2008.
  • 2006
    • C. Chazaud and J. Rossant, “Disruption of early proximodistal patterning and AVE formation in Apc mutants.”, Development, vol. 133 (17) , pp. 3379–87, 2006.
    • C. Chazaud, Y. Yamanaka, T. Pawson and J. Rossant, “Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway.”, Dev. Cell, vol. 10 (5) , pp. 615–24, 2006.
  • 2003
    • J. Rossant, C. Chazaud and Y. Yamanaka, “Lineage allocation and asymmetries in the early mouse embryo.”, Philosophical transactions of the Royal Society of London. Series B, Biological sciences, vol. 358 (1436) , pp. 1341–8; discussion 1349, 2003.